Products

Blood gDNA Extraction Kit(SpinColumn,5-10mL,2-5mL,1-2mL)

Packing Specification：

Product Introduction:
The unique binding buffer/Proteinase K rapidly lyses cells and inactivates intracellular nucleases. Genomic DNA then selectively binds to the silica membrane in the spin column under high chaotropic salt conditions. Following a series of rapid wash-centrifuge steps, cellular metabolites, proteins and other impurities are removed by inhibitor removal buffer and wash buffer. Finally, pure genomic DNA is eluted from the silica membrane with low-salt elution buffer.

Product Features:
1.No toxic reagents such as phenol are used, and ethanol precipitation is not required.
2.Fast and convenient: single-sample processing can generally be completed within 20 minutes.
3.Multiple column washing steps ensure high purity. Typical yield:100–180 µg genomic DNA can be extracted from 3 mL whole blood, with a typical OD₂₆₀/OD₂₈₀ ratio of 1.7–1.9 and fragment length up to 30–50 kb. The purified DNA can be directly used in PCR, Southern blot and various restriction enzyme digestion reactions.
4.The red blood cell lysis buffer is optimized from over a dozen formulations, enabling rapid and complete lysis; customers may purchase it according to their needs.
Typical yield: 100–180 µg genomic DNA can be extracted from 3 mL whole blood.