Products

Urine gDNA Extraction Kit(SpinColumn)

Packing Specification：

Product Introduction：
DNA in urine is derived from exfoliated cells in the urinary tract. Using urine DNA for basic molecular biology research and clinical diagnosis has many special advantages: 1. Urine collection is non-invasive and non-traumatic. 2. Extracting DNA from urine is simpler than extracting it from blood. This product is specifically designed for extracting genomic DNA from urine, and the extracted DNA can be directly used for PCR reactions.

Product Features：
1.Typically, 3-6 µg of genomic DNA can be extracted from 200 μL of whole blood (reference for yield comparison).
2.Simple operation; the entire process is performed at room temperature in approximately 20 minutes, suitable for large-scale sample processing.
3.DNA yield: generally 50-200 ng/mL urine for females and 3-50 ng/mL urine for males.
4.High-purity extracted DNA, directly applicable for PCR, DNA methylation identification, cancer detection, etc.
5.Safe and non-toxic: the kit is non-toxic to the human body and has no corrosive or irritating odor.
6.Cost-effective: equivalent in quality to similar foreign products but more affordable.

Application Scope：
Suitable for the rapid extraction of genomic DNA from urine samples.

Operation Steps：
Tip: Before first use, add the specified amount of anhydrous ethanol to Wash Buffer WB, mix thoroughly, and mark the box to indicate ethanol has been added to avoid repeated addition!

1.Take 5-50 mL of urine into an appropriately sized centrifuge tube. Centrifuge at 3,000 rpm to collect the cell pellet.

2.Carefully discard the supernatant, add 200 μL of Buffer UB to resuspend the cells.

3.Add 20 μL of Proteinase K (20 mg/mL) solution, mix thoroughly. Add 200 μL of Binding Buffer CB, immediately vortex to mix well, and incubate at 70℃ for 10 minutes. The solution should become clear.

4.After cooling, add 100 μL of isopropanol and immediately vortex to mix thoroughly (flocculent precipitation may occur).
Immediate vortexing or pipetting to mix thoroughly is critical in this step. Inadequate mixing will severely reduce yield. For viscous samples that are difficult to mix, vortex for 15 seconds if necessary.

5.Load the mixture (including any precipitation) into a Spin AC column (placed in a collection tube). Centrifuge at 13,000 rpm for 30-60 seconds and discard the waste liquid in the collection tube.

6.Add 500 μL of Inhibitor Removal Buffer IR, centrifuge at 12,000 rpm for 30 seconds, and discard the waste liquid.

7.Add 500 μL of Wash Buffer WB (ensure anhydrous ethanol has been added!), centrifuge at 12,000 rpm for 30 seconds, and discard the waste liquid.

8.Add 500 μL of Wash Buffer WB, centrifuge at 12,000 rpm for 30 seconds, and discard the waste liquid.

9.Place the Spin AC column back into the empty collection tube and centrifuge at 13,000 rpm for 2 minutes to thoroughly remove residual Wash Buffer (residual ethanol may inhibit downstream reactions).

10.Remove the Spin AC column and place it into a clean centrifuge tube. Add 30 μL of Elution Buffer EB (preheating the elution buffer in a 65-70℃ water bath beforehand improves elution efficiency) to the center of the adsorption membrane. Incubate at room temperature for 3-5 minutes, then centrifuge at 12,000 rpm for 1 minute. Transfer the eluted solution back into the spin column, incubate at room temperature for 2 minutes, and centrifuge at 12,000 rpm for 1 minute.
Larger elution volumes result in higher elution efficiency. For higher DNA concentration, reduce the elution volume (minimum 15 μL; volumes smaller than this reduce elution efficiency and DNA yield).

11.Store DNA at 2-8℃ for short-term use or -20℃ for long-term storage.